Composite

Part:BBa_K1867023:Design

Designed by: Shien Yang Lee   Group: iGEM15_NYU-AD   (2015-09-16)


pro_LldD_term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 777
    Illegal BamHI site found at 816
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Had to sequence optimize to remove Biobrick restriction sites from sequence.


Source

Coding sequence from Ecocyc database, accessed at: http://biocyc.org/ECOLI/sequence?object=EG11963

References